T4323
Sigma
Tris-Borate-EDTA buffer
10× concentrate, Biotechnology Performance Certified
| Synonym: | TBE buffer |
| MDL number: | MFCD00236358 |
Description
| Application | TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis.1, TBE is used with non-denaturing or denaturing (7M urea) gels. It is also routinely used for DNA automated sequencing gel.2 TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution. |
| Caution | TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance. |
| Preparation Note | Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134). |
Properties
| grade | Biotechnology Performance Certified |
| sterility | sterile; 0.2 μm filtered |
| total impurities | DNase, RNase, Protease, none detected |
| bioburden, tested | |
| endotoxin, tested | |
| ≤5 ppm heavy metals (as Pb) |
Safety
| Safety Statements | 23-24/25 |
| WGK Germany | 2 |
References
| Cited Reference | 1. Sambrook, J., et al. Molecular Cloning: A Laboratory Manual 2nd ed., Cold Spring Harbor, , (1989), 6.4 |
| 2. Karger, B.L, et al., Capillary electrophoresis with polymer matricies: DNA and protein separation and analysis. Meth. Enzymol. 271, 293-319, (1996) | |
| reference | Ogden, R.C., and Adams, D.A., Electrophoresis in agarose and acrylamide gels. Meth. Enzymol. 152, 61-87, (1987) |
